<div><p>(A) Immunocompetent (BALB/c) mice bearing JC tumors were treated via a single tail vein injection with 1 × 10<sup>8</sup> PFU of vaccinia strains WR or <i>WR-delB18R</i>. Viral titer (PFU/g) in indicated tissues was determined by plaque assay after sacrifice of animals at indicated time points (<i>n</i> = 3 animals/time point).</p>
<p>(B) Immunohistochemistry staining for vaccinia coat proteins in tumor tissue sections of animals treated as above and sacrificed 24 h after viral treatment (magnification 40×).</p>
<p>(C) Survival of immunocompetent mice (C57/B6) bearing subcutaneous CMT-93 tumors and treated when tumors reached 50–100 mm<sup>3</sup> with a single intratumoral (left) or intravenous (right) injection of <i>WR-delB18R</i> or PBS. Doses of 1 × 10<sup>4</sup> (circles), 1 × 10<sup>6</sup> (diamonds), or 1 × 10<sup>8</sup> (triangles) PFU of virus or PBS controls (squares) were used (<i>n</i> = 5 mice/group; <i>p</i> = 0.0047 for intravenous injections).</p></div
