In Vitro Testing of <i>JX-795</i> (Western Reserve Vaccinia Virus Containing Deletions of <i>B18R</i> and <i>Thymidine Kinase</i> Genes and Expressing Luciferase and mIFN-β)

Abstract

<div><p>(A) mIFN-β production following infection of human A2780 cells with <i>JX-795</i> at an MOI of 5.0. IFN-β secretion into the medium during each hour postinfection (up to 14 h) was determined by ELISA.</p> <p>(B) Replication of <i>JX-795</i> (solid symbols and lines; ΔB18RΔTK-IFNb) and an equivalent virus (<i>B18R</i>- and <i>TK</i>-deleted, expressing luciferase only; Δ18ΔTK: open symbols; dashed lines) following infection of murine tumor (CMT-64 [squares] and JC [diamonds]) or nontransformed (NIH 3T3; triangles) cells at an MOI of 1.0.</p> <p>(C) Viral gene expression (luciferase) as measured by bioluminescence 24 h after infection of indicated cell lines with indicated viruses at an MOI of 1.0, and quantified (average of three experiments).</p></div