<i>jagged2a</i>-Expressing Cells in the Pronephric Duct are Multi-Cilia Cells

Abstract

<div><p>(A–D) <i>rfx2</i> is expressed uniformly in the IM at (A) 15 ss, and expression is restricted to individual cells in the pronephric duct from (B) 17 ss onwards to (C) 20 ss and (D) 24 hpf. Arrows demarcate the distal duct region that contains <i>rfx2</i>-expressing cells.</p><p>(E–J) Fluorescent double in situ hybridization of (E and H) <i>jagged2a</i> and (F and I) <i>rfx2</i> revealed that they are (G and J) colocalized in the distal pronephric duct of (E–G) 17-ss and (H–J) 22-hpf embryos. Arrows point to the cells that express <i>jagged2a</i> and <i>rfx2,</i> and arrowhead points to the cells that express <i>jagged2a</i> only.</p><p>(K–M) Fluorescent double in situ hybridization of (K) <i>rfx2</i> and (L) <i>her9</i> revealed that they are expressed in the (M) alternate cells in the distal pronephric duct of 20-ss embryos. Arrowheads point to the <i>rfx2</i>-expressing cells and arrows point to the <i>her9</i>-expressing cells<i>.</i></p><p>All embryos, anterior to the left. (A) and (B) are dorsal views; (C–M) are lateral views. Bar scale: 135 μm (A), 150 μm (B), 100 μm (C), 120 μm (D), and 50 μm (E–M).</p></div