Dynamic Expression of Notch Components in the IM and Pronephric Duct

Abstract

<div><p>(A–D) <i>notch1a</i> (A) and <i>notch3</i> (C) are expressed in the IM at 10 ss. <i>notch1a</i> (B) is expressed in the distal duct region from somite 10 to 14 (see also <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0030018#pgen-0030018-sg003" target="_blank">Figure S3</a>B) at 18 ss, and <i>notch3</i> (D) is expressed in the whole duct from somite 3 to 20 at 24 hpf as indicated by the arrows.</p><p>(E–G) <i>jagged2a</i> expression in the IM appears gradually from anterior to posterior from 5 ss (E) (as indicated by the arrow) to 10 ss (F), and reaches the posterior by 15 ss (G).</p><p>(H–K) <i>jagged2a</i> expression is higher in some cells (arrows point to these cells in [I], which is magnified from [H]) than in neighboring cells in the distal duct at 17 ss (H and I), and transcription is limited to individual cells from 20 ss (J), to 24 hpf (K), to at least 36 hpf (unpublished data) in the demarcated region from somite 8 to 14 (see also <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0030018#pgen-0030018-sg003" target="_blank">Figure S3</a>D and <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0030018#pgen-0030018-sg003" target="_blank">S3</a>F) as indicated by arrows.</p><p>(L and M) <i>her9</i> is expressed in the distal pronephric duct at 18 ss (L) from somite 10 to 12 (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0030018#pgen-0030018-sg003" target="_blank">Figure S3</a>H) and at 21 hpf (M). The arrowhead marks the glomerulus, and arrows demarcate the <i>her9</i> expression region. Left and right inserts in (M) are the magnified images in the glomerulus and distal duct, respectively.</p><p>All embryos, anterior to the left. (A), (C), and (E–I) are dorsal views; the rest are lateral views. Bar scale: 200 μm (A, C, and E), 110 μm (B and D), 180 μm (F), 230 μm (G and H), 90 μm (I), 115 μm (J), 190 μm (K), and 100 μm (L and M).</p></div