RNA Synthesis Phenotype of the Alb <i>ts</i>16 and LA <i>ts</i>6 Mutants

Abstract

<p>RNA synthesis (A) or negative-strand RNA synthesis (B) was determined using 20 or 30 min pulse labels with ³H-uridine in the presence of dactinomycin, with or without the addition of CH, after shifting the incubation temperature of MHV-A59-, Alb <i>ts</i>16-, and LA <i>ts</i>6-infected cells from 30 °C to 40 °C at 8 hpi: filled bar, 0–20 min pulse; grey bar, 20–40 min pulse; open bar, 40–60 min pulse; dark diagonal bar, 0–30 min pulse; light diagonal bar, 30–60 min pulse.</p