Distribution of the Complexes
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Abstract
<p>Cells were seeded into 6-cm plates at a density of 300,000 cells in 2 ml of medium per plate and grown overnight. Cells were then transfected with the fluorescently labeled poly IC (5 μg/ml) using the indicated conjugates. After 4 h, cells were analyzed by fluorescent microscopy (<a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030006#s2" target="_blank">Methods</a>) for selectivity of the transfection (A) and intracellular distribution of the complex in the U87MGwtEGFR cells (B).</p