Structure Models of the Arp2/3 Complex at Actin Branch Junction

Abstract

<div><p>Color codes used: Arp2 (light pink), Arp3 (orange), Arc40/ARPC1 (green), Arc35/ARPC2 (cyan), Arc19/ARPC4 (blue), Arc18/ARPC3 (dark pink), and Arc15/ARPC5 (yellow). Gray arrows indicate the mother and daughter filaments.</p> <p>(A) Orientation of the Arp2/3 complex relative to the mother and daughter filaments as determined using the labeling constraints.</p> <p>(B) Model rotated vertically anticlockwise by 90° from view in (A) and tilted so that the mother filament coincides with the vertical axis. The gray arrow is positioned to pass through the center of the complex.</p> <p>(C) Model rotated vertically by 180° from the view in (A).</p> <p>(D) Label positions and their corresponding C-termini localization. GFP or YFP, shown as ribbon diagram with the same color coding as in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0030383#pbio-0030383-g003" target="_blank">Figure 3</a>, were superimposed on the respective difference peak (<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0030383#pbio-0030383-g003" target="_blank">Figure 3</a>) with their orientation matching the peak shape.</p> <p>(E) Model superimposed on the projection density map (white corresponds to high density).</p> <p>(F) Model and ribbon diagram of a daughter filament (white) as it would grow after small relative rotations of Arp2 and Arp3 (see text) superimposed on the projection density map.</p> <p>(G and H) Model proposed by Beltzner and Pollard [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0030383#pbio-0030383-b06" target="_blank">6</a>] (G) and by Aguda et al. [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0030383#pbio-0030383-b05" target="_blank">5</a>] (H) shown for comparison. Note that in (G), the daughter filament will be oriented out of the paper plane toward the reader.</p> <p>(I)Arp2/3 crystal structure in the same orientation as originally presented in Robinson et al. [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0030383#pbio-0030383-b12" target="_blank">12</a>].</p></div

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