Visualization of NPFs in the Actin Branch Junction by Difference Mapping

Abstract

<div><p>(A-F) WASp protein dissociate from the actin branch. 2D average projection obtained (A) using the unlabeled yeast Arp2/3 complex and N-WASp WA, and (B) using the full-length GST-N-WASp complexed with its activator GST-Nck. (C) Difference map between (A) and (B). 2D average projection obtained using (D) the amoeba Arp2/3 complex and Scar WA, and (E) MBP-Scar1WA. (F) Difference map between (D) and (E).</p> <p>(G-I) Cortactin is present in the actin branch. (G) 2D average projection obtained using GST-cortactin and the amoeba Arp2/3 complex. (H) Difference map between (G) and (D). (I) The peak in the difference map shown in yellow superimposed with the projection map.</p> <p>Bar = 10 nm.</p></div

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