Effect of curcumin and/or 5-FU on mitochondrial damage and cytochrome c release in HCT116 and HCT116+ch3 colon cancer cells.

Abstract

<p>HCT116 cells were treated with 20 µM curcumin or 5 µM 5-FU or a combination of 5 µM curcumin (4 h pretreatment) and 1 µM 5-FU for 24 h. HCT116+ch3 cells were treated with 5 µM curcumin or 1 µM 5-FU or a combination of 5 µM curcumin (4 h pretreatment) and 0.1 µM 5-FU for 24 h. Mitochondrial and cytoplasmic cell fractions were prepared and analyzed by western blotting using antibodies against cytochrome c. The housekeeping protein β-actin served as a loading control.</p

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