Ultrastructural evaluation of mitochondrial and apoptotic changes after treatment with curcumin and/or 5-FU in HCT116 and HCT116+ch3 colon cancer cells.

Abstract

<p> HCT116 cells were treated with curcumin (20 µM), 5-FU (5 µM) or a combination of both (5 µM curcumin and 1 µM 5-FU) for 12, 24, 36, 48, 60 and 72 h. Using a different approach HCT116+ch3 cells were treated with a combination of 5 µM curcumin and 0.1 µM 5-FU for 12, 24, 36, 48, 60 and 72 h. Ultrathin sections were prepared and evaluated by transmission electron microscopy. Micrographs shown are representative of all the cultures evaluated. At the earliest time point when apoptosis was first detected images are highlighted arrows. Mitochondrial changes (arrowheads) are shown. Magnification: x5000, bar = 1 µm.</p

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