Effect of curcumin and/or 5-FU on apoptotic signaling in HCT116 and HCT116+ch3 colon cancer cells.

Abstract

<p>HCT116 cells were treated with 20 µM curcumin or 5 µM 5-FU or a combination of 5 µM curcumin (4 h pretreatment) and 1 µM 5-FU for 24 h. HCT116+ch3 cells were treated with 5 µM curcumin or 1 µM 5-FU or a combination of 5 µM curcumin (4 h pretreatment) and 0.1 µM 5-FU for 24 h. Whole cell lysates were prepared and analyzed by western blotting for <b>A:</b> expression or cleavage of pro-apoptotic proteins caspase-8, caspase-9, caspase-3, PARP and Bax, and of anti-apoptotic protein BCL-xL <b>B:</b> expression of cyclin D1. The housekeeping protein β-actin served as a positive loading control in all experiments.</p

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