Effect of curcumin and/or 5-FU on NF-κB and PI-3K/Src activation in HCT116 and HCT116+ch3 colon cancer cells.


<p>HCT116 and HCT116+ch3 cells were either treated with different concentrations of 5-FU (0, 2, 5, 10, 20, 40 µM) alone for 1 h or were pretreated with different concentrations of curcumin (0, 2, 5, 10, 20, 40 µM) for 1 h and then exposed to 1 µM (HCT116) or 0.1 µM (HCT116+ch3) 5-FU for 1 h. <b>A:</b> After preparation of nuclear extracts western blotting was performed with antibodies against NF-κB and PARP as a loading control. <b>B:</b> Cytoplasmic fractions were subsequently examined by western blotting for expression of PI-3K (lane I), Src (lane II) and β-actin (lane III) (loading control).</p

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