<p>The kinetic parameters of the recombinant SmIDH were determined by measuring its enzyme activity at various isocitrate or NAD<sup>+</sup> concentrations with the other substrate at saturating concentrations. Enzymatic activity was assessed by monitoring the increase of NADH. The SmIDH <i>K</i><sub>m</sub> for NAD<sup>+</sup> (A) and isocitrate (B) were calculated as 154 μM and 75 μM, respectively, by averaging values from triplicate experiments.</p
