Abstract

<p>(A) Immunofluorescence analysis of intracellular parasites stably expressing ddFKBPmyc-Rab5A(N158I) treated for 0, 12, 18 and 24 hrs with 1 µM Shld-1 and co-stained with the indicated microneme antibodies (green/red) and Dapi (blue). (B) Quantification of localisations of Rop2-4 and indicated microneme proteins. 300–400 PVs of three independent experiments were analysed and normalised with RH <sup>hxgprt−</sup>parasites. Mean values and the respective standard deviation are presented. Note that MIC2 and M2AP is mainly mislocalised inside the parasite (arrowhead in A).</p

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