Abstract

<p>(A) Growth analysis of parasites expressing indicated ddFKBPmyc-Rab constructs inoculated on HFF cells and cultured for 5 days +/− 1 µM Shld-1. Single plaques are indicated by black edging. The scale bar represents 1 mm. The depicted quantification of the plaque sizes is a representative of 3 independent experiments. In each case, the mean area and standard deviation of 10 plaques was determined. The data was normalised relative to the plaque size of the respective uninduced parasite strain. (B) Immunofluorescence analysis of intracellular parasites expressing indicated ddFKBPmyc-Rab constructs and wild type parasites RH<sup>hxgprt−</sup>treated for 24 hrs with 1 µM Shld-1 and probed with α-MIC3, α-MIC2 or α-ROP2-4 antibody (red) and Dapi (blue). Only parasites overexpressing Rab5A and 5C show a mislocalisation effect on the secretory organelles (micronemes, rhoptries). Scale bars represent 5 µm.</p

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