Abstract

<p>(A) Parasites expressing ddFKBPmyc-Rab5A(N158I) have been grown for 24 hrs +/− 1 µM Shld-1 and analysed with the indicated antibodies. MIC3 and MIC11 are not transported to the micronemes, whereas the micronemal proteins PLP1 and M2AP as well as the marker for endosomal-like compartments (CPL and TgVP1) show normal localisation. The scale bars represent 5 µm. (B,C) Pulse-chase experiments of MIC3 processing in parasites expressing ddFKBPmyc-Rab5A(N158I). (B) Immunofluorescence analysis of ddFKBPmyc-Rab5A(N158I) parasites grown for 18 hrs +/− 1 µM Shld-1 and probed with proMIC3 (red) and CPL (green) antibodies. In presence of the inducer the pro-peptide of MIC3 is secreted into the PV (proMIC3), whereas no effect on endosomal-like compartments (CPL) is obvious. Dapi is shown in blue. The scale bar represents 5 µm. (C) Quantification and the respective western blots of MIC3 maturation in RH <sup>hxgprt−</sup> and ddFKBPmyc-Rab5A(N158I) parasites are shown. Mean values and the respective standard deviation of three independent experiments are presented.</p

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