<p>Representative chromatograms of pure paclitaxel and 17-AAG (green trace), an extract of drug-spiked liver tissue (blue trace) and a blank liver extract (black trace) are shown at 221 nm (A), 333 nm (B) and 281 nm (C), while the peaks of interest at each detection wavelength are shown in red. The retention time of paclitaxel, 17-AAG and α-naphthoflavone was 27.6 min, 36.8 min and 39.3 min, respectively. The concentration of paclitaxel and 17-AAG was both 10 µM, and α-naphthoflavone was used at 20 µM as an internal standard.</p
