Caspase-3 activation in E32511 model mouse brain.

Abstract

<p>Lesions were not detected in the brains of control (uninfected), whereas lesions were detected in the brains of E32511-infected. Scale bars: 1.5 mm. (A) Caspase-3 activation was detected in the neurons of the mouse spinal cord. Scale bars: 750 µm and 200 µm (inset). (B) Caspase-3 activation was also detected in neurons of the mouse reticular formation, scale bars: 300 µm and 200 µm (inset) and endothelial cells of the midbrain. Scale bars: 25 µm. (C) Four week-old female ICR outbred mice after inoculation. The representative clinical features of flaccid paralysis of the extremities were observed in ICR mice 3 to 5 days after orogastric challenge with E32511 10¹¹ CFU/0.5 mL per mouse (green arrow). (D) Four week-old female ICR mice after inoculation. The representative clinical features of spinal deformity were observed in ICR mice 3 to 5 days after orogastric challenge with E32511/HSC 10¹¹ CFU/0.5 mL per mouse (green arrow).</p