Identification of novel HIV-1 in Bulgaria.

Abstract

<p>Phylogenetic relationships were inferred using Bayesian methods implemented in the program BEAST using a relaxed molecular clock and constant population size tree prior. Antiretroviral resistance-associated mutations were stripped from the alignment. Posterior probabilities >0.6 are provided at the nodes. The scale bar indicates relative units of time. Nearly identical tree topologies were also obtained with maximum likelihood analysis. (<b>A</b>) <b>F-like sub-subtypes.</b> The 758-bp alignment was composed of 9 subtype F and F-like sub-subtype polymerase sequences from Bulgaria and 43 subtype F reference sequences from the Los Alamos HIV database and highly related sequences identified using BLAST. The tree was rooted by using three HIV-1 subtype J strains as the outgroup. (<b>B</b>) <b>A-like sub-subtypes.</b> The 754-bp alignment consisted of 10 subtype A and A-like sub-subtype polymerase sequences from Bulgaria and 121 subtype A reference sequences from the Los Alamos HIV database and highly related sequences identified using BLAST. Antiretroviral resistance-associated mutations were stripped from the alignment. The tree was rooted by using three HIV-1 J strains as the outgroup.</p