<p>Cells from naïve or 5 week infected draining lymph nodes or from ears were analyzed for IFN-γ, IL-17A and IL-4 production. Antigen-specific cytokine release by draining lymph node cells from 5 week infected mice was determined by ELISA after restimulation with <i>L. major</i> freeze-thawed antigen (FTAg). Values represent mean ± SEM of 5 mice per group (ND: not detectable) (A). Cells from the ears of 5 week infected mice were stimulated with PMA and ionomycin for 4 h and analyzed for intracellular cytokine production. Contour plots and bar graphs show the frequency and number of IFN-γ (B) and IL-17A (C) in gated CD4 T cells at 1 (top) and 5 (bottom) weeks after infection. Frequency of double positive IL-17A/IL-10 (D) or IL-17A/IFN-γ (E) producer CD4 T cells at 1 or 5 weeks after infection, respectively. Quadrant values are the percentages from total gated population. Numbers between parentheses indicate the mean fluorescence intensity (MFI) for the IL-17 within the CD4 T cells. The data shown are from one experiment and are representative of at least three experiments (*, <i>p</i><0.05).</p
