Results of <i>Arabidopsis thaliana</i> Sanger sequencing.

Abstract

<p>From the ranked list of all SNPs predicted by Bubbleparse in contigs of over 200 nt, the top 48, as well as 16 from 25%, 50% and 75% down the list were tested with Sanger sequencing. This confirmed all but 5 as being real SNPs between Col-0 and Ler-1. The remaining five all had sequencing problems – such as the sequence ending before the SNP was reached – so are not confirmed as false postives.</p