The time course of NF-κB DNA-binding activity detected by EMSA after SAH and the effects of PDTC on NF-κB activation.

Abstract

<p>A. The representative autoradiogram showed the NF-κB DNA-binding activity in each group. B. The time course of NF-κB DNA-binding activity after SAH. Quantification of the DNA-binding activity of NF-κB was performed by densitometric analysis. The NF-κB DNA-binding activity was up-regulated significantly after SAH, especially on day 3 and 5, while restored on day 7. C. the effects of intracisternal administration of PDTC on NF-κB DNA-binding activity. It was shown that NF-κB DNA-binding activity was suppressed after treatment with PDTC. 1 stands for the control group. 2, 3 and 4 stand for the D-3, 5 and 7 SAH groups. 5 stands for the PDTC groups. Results are represented as means ± SEM from five independent experiments in each group. *P<0.05 vs. control group, **P<0.01 vs. control group, #P<0.05 vs. D-5 SAH group.</p