Enhancement of HIV-1 infection of SEVI in the presence of polyanions.

Abstract

<p>Fibril formation of PAP248-286 was promoted by agitation in the presence of polyanions (30 µg/ml) or PBS for 60 h. PAP248-286 at different concentration were mixed with R5 tropic (A) and X4 tropic viruses (B), respectively. The mixtures were then added to TZM-b1 cells. After culture at 37°C for 3 h, the medium was replaced. Luciferase activity was detected 72 h later. Experiments were repeated once and similar results were obtained. Shown are average values (± standard deviations) of triplicate measurements of a representative experiment.</p