Sequence-specific seeding of RΔ2-5.

Abstract

<p>(A, B) Confocal images of (A) animals expressing GFP-tagged RΔ2-5i::GFP in intestinal cells and (B) 24 h after the injection of Alexa-555-tagged (red) recombinant Sup35NM fibrils. Boxed areas in A and B are representative regions subjected to FRAP analysis. (C) Enlargement of overlay picture in B. (D) FRAP analysis of RΔ2-5i::GFP control (1) and RΔ2-5i::GFP aggregates (2) formed after injection of recombinant Sup35NM fibrils. RFI = relative fluorescence intensity in [%]. (E) Confocal images of animals expressing RΔ2-5i::GFP in the intestine 24 h after injection of the indicated recombinant fibrils.</p