<p>(A) Phosphoinositides plays a central role in the synthesis of sphingolipids in yeast. Gene names are shown in italics. Metabolic intermediates and complex sphingolipids are shown in bold font. Inactivation of <i>KES1</i> inhibits the synthesis of sphingolipids. (B) Cells were grown to late logarithmic phase and inoculated into medium containing <i>myo</i>-[<sup>3</sup>H]inositol, grown to mid-logarithmic phase and lipids were extracted and resolved by thin layer chromatography. Radioactivity of the resolved lipids was measured for PI, IPC, MIPC and M(IP)<sub>2</sub>C. Values were normalized to cell number and are presented as fold-change over wild type. Data represent mean +/− SE of four independent experiments performed in triplicate. *P<0.05 respective to control.</p
