miR-34a enhanced the growth inhibitory effect of c-MET targeting agents in HCC HepG2 cells.

Abstract

<p>HepG2 cells (2.5×10³ cells per well in 96-well-plate) were treated with miR-34a mimic with c-MET siRNA (A) or su11274 (B). MTS was performed as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061054#pone-0061054-g002" target="_blank">Figure 2</a> and the proliferation inhibition rate was calculated. Bliss independence criterion was performed to calculate the theoretical additive effect. (C) Caspase-3/7 activity. * <i>P</i><0.05, ** <i>P</i><0.01, compared to agent alone. (D) c-MET protein level with single or dual treatments detected by western blot. Antibodies include: c-MET and β-actin.</p