Effect of miR-34a on apoptosis in HCC cells.

Abstract

<p>HepG2, HepB3 and SNU449 cells were treated as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061054#pone-0061054-g002" target="_blank">Figure 2</a>. (A) Caspase-3/7 activity was detected using Apo-ONE Homogeneous Caspase-3/7 Assay. * <i>P</i><0.05, ** <i>P</i><0.01, compared to blank and negative controls at the same time point. (B) Hoechst 33342/PI double fluorescent chromatin staining with different transfections for 96 hrs in HepG2 cells (×200). (C) Western blot and signal intensity of the bands. Cells were treated for 96 h. Antibodies include: full length caspase-3, cleaved caspase-3 and β-actin. M: mock control; C1: Negative control for miRNA inhibitor; Inhi: miR-34a inhibitor; C2: Negative control for miRNA mimic; Mimi: miR-34a mimic.</p