MMP-9 mediates invasion and spread of CD133⁺ U87 GSCs in zebrafish embryos.

Abstract

<p>A. The MMP-2 and MMP-9 RNA in CD133⁻ U87 cells and CD133⁺ U87 GSCs were examined by qRT-PCR. B. The MMP-2 and MMP-9 proteins in CD133⁻ U87 cells and CD133⁺ U87 GSCs examined by Western blot. C. The inhibitory effect of MMP-9 inhibitor (AG-L-66085) on the invasion of CD133⁺ U87 GSCs within zebrafish embryos. The embryos xenografted with CD133⁺ U87 GSCs were treated with 2 µM AG-L-66085 or DMSO control. The percentages of invasive cells in injected embryos (low, medium, or high-invasion) were measured at 2 dpi. The data were obtained from three replicate experiments with the number of embryos: n = 123 for DMSO control group, n = 119 for MMP-9 inhibitor group, and n = 144 for negative control group (<i>p</i><0.001).</p