Primers for the multiplex tetra-primer ARMS-PCR for SNPs associated with breast and gynecologic cancers.

Abstract

a<p>Underlined is the universal tag sequence at the 5′ end of the chimeric primers.</p>b<p>Allele-specific nucleotides at the 3′ end are indicated in bold letters.</p>c<p>Specificity is increased by the introduction of a deliberate mismatch at position -1 of the polymorphism site, indicated by bold lower case letters.</p>d<p>A pair of universal primers annealing to the 5′ portion of each chimeric primer.</p