Aft2 is required for the oxidative stress response.
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Abstract
<p>(A) Overnight cultures of each strain were resuspended in YPD medium with OD<sub>600</sub> of 0.1. 5 ul of cells in 10-fold serial dilutions were spotted onto solid YPD or YPD +1 mM Fe<sup>3+</sup> medium that was supplemented with the indicated concentrations of H<sub>2</sub>O<sub>2</sub>. The plates were incubated at 30°C for 2 days and photographed. (B, C) Reactive oxygen species (ROS) and superoxide dismutase enzymes (SOD) activity were measured as described in Materials and methods.</p