Effects of Cry1 on cell growth.

Abstract

<p>(<b>A</b>) Cry1 protein levels were upregulated in HCT116 cells and downregulated in SW480 cells. (<b>B</b>) MTT assays on HCT116 cells 24, 48, and 72 h after transfection with Cry1 or GFP control (***, p<0.001). (<b>C–D</b>) Colony formation assay of HCT116 cells transfected with Cry1 or GFP control (*, <i>p</i> = 0.033). (<b>E–F</b>) Inhibition of SW480 cell colony formation capacity by Cry1 siRNA relative to control (**, <i>p</i> = 0.007). Experiments were repeated at least three times, and representative data are presented; <i>bars</i>, SD.*, <i>P</i><0.05; **, <i>P</i><0.01, ***, <i>p</i><0.001.</p