Activated (CD25⁺ CD69⁺) memory CD4⁺ T-cells have elevated expression of pSer175 CDK9 and CycT1.

Abstract

<p>(A) Resting memory CD4⁺ T-cells isolated from a healthy donor stained for flourophore-conjugated antibodies against the T-cell activation markers (CD25 and CD69) and P-TEFb components (Cyclin T1 and pSer175 CDK9) components and then analyzed by multicolor flow cytometry. (B) Cells from the same donor activated for 16 hr with α-CD3 and α-CD28 mAbs.</p