Intracellular Ca²⁺ and intracellular Ca²⁺ regulatory protein properties in hearts from low fat (LF) or high fat (HF)-fed C57 mice with or without TUDCA treatment (300 mg/kg for 15 days).

Abstract

<p>A: Representative traces depicting intracellular Ca²⁺ transients in LF and HF-fed mice; B: Representative traces depicting Ca²⁺ transients in LF and HF-fed mice with TUDCA treatment; C: Baseline intracellular Ca²⁺ levels; D: Rise in intracellular Ca²⁺ in response to electrical stimulus shown as changes in Fura-2 fluorescence intensity (ΔFFI); E: Single exponential intracellular Ca²⁺ decay rate; F: Representative gel blots depicting levels of SERCA2a, Na⁺-Ca²⁺ exchanger (NCX), total/phosphorylated phospholamban (pPLB) and α-tubulin (loading control) using specific antibodies; G: SERCA2a expression; H: NCX expression; and I: pPLB (Ser¹⁶)-to-PLB ratio. Mean±SEM, n = 44–47 cells from 3 mice (panels A-E); or denoted in the graphs (panels F-I); *p<0.05 (two-way ANOVA).</p