qRT-PCR analysis of sX13-regulated genes.

Abstract

<p>Selected sX13-regulated genes (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003626#ppat-1003626-t001" target="_blank">Table 1</a>) were analyzed by qRT-PCR using RNA from NYG- and MMA-grown <i>Xcv</i> strains 85-10 (wt) and Δ<i>sX13</i>. The amount of each mRNA in the wt was set to 1. Bars represent fold-changes of mRNA amounts in strain Δ<i>sX13</i> compared to 85-10 on a logarithmic scale (log₁₀). Data points and error bars represent mean values and standard deviations obtained with at least three independent biological samples. Asterisks denote statistically significant differences (<i>t</i>-test; <i>P</i><0.05). Dashed lines indicate a 1.5-fold change. Transcripts not detected in the microarray analyses are marked with ‘a’.</p