<p>The entry vectors differ only in the overhangs that are created by BsaI restriction digest. These overhangs define the position of the fragment in the final assembly. Defined amino acid linker sequences are retained, since the overhangs as well as parts of the subcloning toolbox are retained in the final assembly. GS/x/GT are linkers introduced by the BamHI, KpnI restriction sites. TA-Cloning via the XcmI sites introduces the SGTA linker. Note compatible overhangs in consecutive entry vectors.</p
