Inhibitory activity of GRFT and its mutant variants against DC-SIGN-mediated virus transmission of HIV-1-captured virions to CD4⁺ T-lymphocyte cells.

Abstract

<p>The HIV-1-captured Raji/DC-SIGN cultures used for virus transmission in the presence or absence of the wild-type and mutant GRFT variants contained following amounts of captured HIV-1: 5.50±2.09 ng p24/10⁶ cells (for the WT GRFT experiments), 4.30±0.15 ng p24/10⁶ cells (for the mutant D30A GRFT experiments), 3.66±0.59 ng p24/10⁶ cells (for the mutant D70A GRFT experiments), 4.04±0.72 ng p24/10⁶ cells (for the mutant D112A GRFT experiments), and 3.78±0.77 ng p24/10⁶ cells (for the mutant Triple A GRFT experiments). Data represent the mean of three independent experiments.</p