<p>Panel A: Effect of GRFT exposure on the HIV-1(III<sub>B</sub>) gp120 complex with DC-SIGN. 200 nM gp120 was injected (time point a), followed after 2 min by an additional injection (time point b) of varying concentrations of GRFT (0.1 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Balzarini1" target="_blank">[1]</a>, 1 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Balzarini2" target="_blank">[2]</a>, 10 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Balzarini3" target="_blank">[3]</a>, 25 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Hladik1" target="_blank">[4]</a>, 100 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Balzarini4" target="_blank">[5]</a> and 250 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Mori1" target="_blank">[6]</a>). Similar experiments are depicted in Panels B and D but for varying concentrations of mutant D30A GRFT variant and mutant D112A GRFT variant (0.2 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Balzarini1" target="_blank">[1]</a>, 2 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Balzarini2" target="_blank">[2]</a>, 20 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Balzarini3" target="_blank">[3]</a>, 50 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Hladik1" target="_blank">[4]</a>, 200 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Balzarini4" target="_blank">[5]</a> and 500 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Mori1" target="_blank">[6]</a>), respectively. In Panel C the concentrations of mutant D70A GRFT variant were 0.4 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Balzarini1" target="_blank">[1]</a>, 4 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Balzarini2" target="_blank">[2]</a>, 40 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Balzarini3" target="_blank">[3]</a>, 100 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Hladik1" target="_blank">[4]</a>, 400 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Balzarini4" target="_blank">[5]</a> and 1000 nM <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064132#pone.0064132-Mori1" target="_blank">[6]</a>. The curves show a representative example out of two independent experiments.</p
