<p>(A–F) Lateral/oblique views of 4-dpf embryos after whole-mount ISH for <i>th</i> (A–C) and <i>dbh</i> (D–F) in control and <i>phox2b</i>–deficient embryos. Arrows indicate the SCG. Knockdown of <i>phox2b</i> by injection of a splice MO (4 ng) (MO<sup>splice</sup>) inhibits the expression of <i>th</i> and <i>dbh</i> (B, E) which is rescued by coexpression of human <i>PHOX2B</i> mRNA (10 ng/µl) (C, F). Relative intensity levels of <i>th</i> (G) and <i>dbh</i> (H) expression in embryos injected with <i>phox2b</i> MOs that inhibit translation (MO<sup>ATG</sup>) or splicing (MO<sup>splice</sup>). Mismatched control MO (MO<sup>mm</sup>) and <i>PHOX2B</i> mRNA-rescue (MO<sup>splice</sup>/<i>PHOX2B</i>) are also shown. Data are presented as means ± SD (<sup>***</sup>P<0.001; <sup>**</sup>P<0.01; n = 15 for each group).</p
