<p>(<b>A</b>) Overexpression of <i>MSB1</i> inhibits the growth of <i>rho1-3</i> cells. Cells of yeast strain NY2284 (WT), NY2285 (<i>rho1-2</i>), and NY2286 (<i>rho1-3</i>) carrying pEGKT316 (Vec) or pEGKT316-MSB1 (GAL-MSB1) were grown in SC-Ura medium containing dextrose (Dex) or galactose and raffinose (Gal) at 30°C. Pictures were taken after 3 d. (<b>B</b>) Overexpression of <i>MSB1</i> does not inhibit the growth of <i>cdc42</i>-Ts cells. Cells of yeast strain YEF473A (WT), YEF2258 (<i>cdc42-201</i>), and JPC241 (<i>cdc42<sup>G60D</sup></i>) carrying pEGKT316 (Vec) or pEGKT316-MSB1 (GAL-MSB1) were grown in SC-Ura medium containing dextrose (Dex) or galactose and raffinose (Gal) at 30°C. Pictures were taken after 4 d. (<b>C</b>) Msb1 interacts with Rho1 by GST pull-down assay. Cells of yeast strain YEF1395 (<i>msb1</i>Δ) carrying YEp181-3HA-MSB1 along with pEGKT (GST), pEGKT-CDC42 (Cdc42), or pEGKT-RHO1 (Rho1) were used in the assay. Molecular weight: GST (27 kDa), GST-Cdc42 (46 kDa), GST-Rho1 (48 kDa).</p
