<p>(<b>A</b>) Overexpression of <i>MSB1</i> inhibits the growth of <i>rho1-104</i> cells. Cells of yeast strain NY1537 (WT) and NY1538 (<i>rho1-104</i>) carrying pEGKT316 (Vec) or pEGKT316-MSB1 (GAL-MSB1) were grown in SC-Ura medium containing dextrose (Dex) or galactose and raffinose (Gal) at 30°C. Pictures were taken after 3 d. (<b>B</b>) Overexpression of <i>MSB1</i> in <i>rho1-104</i> cells causes the accumulation of small-budded cells. The percentage of small buds in the population of budding cells overexpressing <i>MSB1</i> as in panel A was quantitated. More than 200 buds were scored. (<b>C</b>) Glucan distribution in <i>rho1-104</i> cells overexpressing <i>MSB1</i>. Cells as in panel A were stained for 1,3-β-glucan. Bar, 5 µm.</p
