<p>(<b>A</b>) Morphology and septin organization (shown by GFP-Cdc3) in cells overexpressing <i>MSB1</i>. Cells of yeast strain JGY881 (<i>GFP-CDC3</i>) carrying pEGKT316 (Vec) or pEGKT316-MSB1 (GAL-MSB1) were grown in SC-Ura medium containing galactose and raffinose at 30°C for 16 h. DIC and GFP fluorescence images were taken. (<b>B</b>, <b>C</b>) Chitin deposition (<b>B</b>) as well as 1,3-β-glucan and mannan distribution (<b>C</b>) were visualized in cells of strain YEF473A carrying pEGKT316 (Vec) or pEGKT316-MSB1 (GAL-MSB1) grown in SC-Ura medium containing galactose and raffinose at 30°C. The cells were stained for chitin, 1,3-β-glucan, and mannan. (<b>D</b>) Msb1 localizes to sites of aberrant glucan deposition. Cells of strain JGY139A (<i>GAL1-GFP-MSB1</i>) was grown in SC-Ura medium containing galactose and raffinose at 30°C. Cells were stained for 1,3-β-glucan with aniline blue. Bars, 5 µm.</p
