Experiment protocols.

Abstract

<p>HUVECs submitted to the anoxia and reoxygenation (A/R) and pretreated with and without 1.0 minimum alveolar concentration (MAC) desflurane or 5 µM BAY11-7082 (BAY). For cell viability, apoptosis, SOD activity assays, the cell samples were collected at the end of experiments. For western blot analysis, cell samples were collected at the end of reoxygenation (samples were collected at 45 min after baseline in CON group and 15 min after desflurane exposure in DES group).</p