Effects of fibronectin and collagen on cytokine production by ECFCs.

Abstract

<p>ECFCs expanded on collagen produced higher levels of IL-6 and IL-8 that ECFCs expanded on fibronectin. (A) Representative curves showing that the concentration of IL-6 and IL-8 in the supernatants of ECFCs expanded on fibronectin (white circles) and collagen (gray circles) tended to increase with increasing passages. (B) ECFCs expanded on collagen (gray bars) reached higher levels of IL-6 and IL-8, but not bFGF, than ECFCs cultured on fibronectin (white bars). The mean ± SEM of the highest cytokine concentration reached during culture of the same 14 ECFC colonies reported in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066734#pone-0066734-g002" target="_blank">Figure 2</a> is shown. p values calculated by the Wilcoxon signed-rank test.</p