Auxin homeostasis in <i>Bdtar2l^hypo</i> roots and its relation to the ethylene pathway.

Abstract

<p>(<i>A</i>) Free auxin (IAA) content in wild type and <i>Bdtar2l^hypo</i> root segments at 4 dag. The root tip comprised the terminal 8 mm of the roots, the elongated parts all above this. (<i>B</i>) Expression levels of the homologs of various genes encoding rate limiting enzymes in alternative auxin biosynthesis pathways in wild type and <i>Bdtar2l^hypo</i> roots at 4 dag. (<i>C</i>) Expression levels of <i>YUCCA</i> homologs in wild type and <i>Bdtar2l^hypo</i> roots at 4 dag. (<i>D</i>) Expression levels of <i>BdTAR1L</i> and <i>BdTAR2L</i> in wild type at 3 dag and after 3 h of ACC treatment. (<i>E</i>) Expression levels of <i>YUCCA</i> homologs in wild type at 3 dag and after 3 h of ACC treatment. All expression levels were determined by qPCR and normalized with respect to the housekeeping gene, <i>BdUBC18</i>; differences as compared to wild type or mock are not significant unless indicated otherwise; error bars indicate standard error; * = p<0.05; ** = p<0.01; *** = p<0.001.</p