Phenotypes of the <i>Bdtar2l^qnull</i> mutant in comparison to its wild type background, Bd21-3.

Abstract

<p>(<i>A</i>) Relative expression level of <i>BdTAR2L</i> in different genotypes at 4 dag as determined by qPCR and normalized with respect to the housekeeping gene, <i>BdUBC18</i>. (<i>B–C</i>) Root elongation in wild type and <i>Bdtar2l^qnull</i> mutants, assayed at 4 dag. (<i>D</i>) Representative Nomarski optics images of mature root portions. Arrowheads point out top and bottom of individual cells in a cortex layer; (<i>E</i>) Quantification of mature cortex cell length at 4 dag. (<i>F</i>) Representative microscopy images of root hairs at 4 dag. (<i>G</i>) Representative light microscopy images of transverse sections across the mature root. (<i>H–I</i>) Quantification of total transverse area and stele area in sections from mature roots. (<i>J</i>) Relative seminal root length in <i>Bdtar2l^qnull</i> mutants during root growth progression. (<i>K</i>) Progressive breakdown of root meristem as indicated by shrinkage of the meristematic zone. (<i>L</i>) Seedling shoot length at 4 dag. (<i>M</i>) Adult shoots. Size bars are 1 cm (<i>B, M</i>) or 100 µm (<i>D,F,G</i>); differences as compared to wild type or mock are not significant unless indicated otherwise; error bars indicate standard error; * = p<0.05; *** = p<0.001.</p