<p>Representative images of a Cy2-stained black bear serum proteins separated by difference gel electrophoresis, utilizing a pH 4–7 (left to right) immobilized pH gradient isoelectric focusing gel strip for the 1<sup>st</sup>, horizontal separation and a 12% SDS-PAGE gel in the 2<sup>nd</sup>, vertical separation. Protein spots significantly (p<0.05) up-regulated (yellow) and down-regulated (green) proteins are indicated by colour. Protein spots that were subsequently identified by tandem mass spectrometry are indicated by numbers, which correspond to spot IDs in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066119#pone-0066119-t002" target="_blank">table 2</a>. Estimated protein molecular weights in kiloDaltons (kDa) are indicated on the right edge of the image, and estimated isoelectric points are on the bottom edge.</p

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