hnRNP K regulates M1 mRNA splicing.

Abstract

<p>(A) Cell extracts from A549 cells transfected with non-targeting siRNAs or siRNAs that target various indicated hnRNPs for 48 h were subjected to immunoblot analysis with antibodies specific to each hnRNP. (B and C) A549 cells were transfected as in B and then infected with A/WSN/33 at MOI 2 for the indicated time points. In C, NS1-BP and hnRNP K were simultaneously knocked down. Total RNA was isolated at each depicted time point and analyzed by real-time RT-PCR with primers specific to M1 and M2 mRNAs. M2/M1 mRNA ratios are shown. Error bars represent mean ± SD (n = 3). *p<0.05, **p<0.01, ***p<0.001.</p