Long-range water accessibility study by ESE.

Abstract

<p>The theoretical fits (red lines) to the ESE experimental data (blue lines) using a stretched exponential function (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068264#pone-0068264-t001" target="_blank">Table 1</a>) as previously described. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068264#pone.0068264-Huang1" target="_blank">[11]</a> The results for the n3β-s and PPm3-s are shown in (a) and (b), respectively. The decay signals acquired by the ESE experiments were fitted over the maxima of the deuterium modulation as described in Zecevic et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068264#pone.0068264-Zecevic1" target="_blank">[32]</a> to minimize the influence from destructive interference of nuclear modulations. The obtained values of the T_M (in ns) and stretching exponent x are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068264#pone-0068264-t001" target="_blank">Table 1</a>. The T_M values can be directly used to yield the surrounding proton density (<i>C_ex</i>; cf. Eq. 2) within the range of ∼2 nm from a nitroxide spin.</p