Water accessibility study of the PPm3-s by ESEEM.
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Abstract
<p>(a) Three-pulse ESEEM time-domain data (solid lines) after the removal of the exponential decaying function in the raw data. The modulation depth is directly correlated to the peak intensity of the FT-ESEEM and can be quantitatively characterized by the best-fit parameter <i>k<sub>D</sub></i> (cf. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068264#pone-0068264-t001" target="_blank">Table 1</a>). The dashed lines represent the theoretical fits to the experimental data using the equation described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068264#pone-0068264-g003" target="_blank">Figure 3</a>. (b) The FT-ESEEM data for the PPm3-s in various deuterated conditions. The peaks correspond to the Larmor frequency of nucleus <sup>2</sup>H, indicating the PPm3-s is surrounded by D<sub>2</sub>O. The inset shows a ribbon model of a PPm3 variant carrying three spin labels.</p
