<p>A). RT-PCR showed expression pattern of <i>Spata33</i> mRNA in testes from P2 to P20 (postnatal days). Total RNAs were isolated from mouse testes and then cDNAs were synthesized. <i>Hprt</i> was used as an internal control. Molecular weight is shown on the right. <i>Spata33</i> mRNA was increased markedly after P12. B). Real-time fluorescent quantitative PCR of the <i>Spata33</i> in testes from P2 to P20. Error bars indicate the standard deviation (SD) of the mean (n = 3). Y-axis represents relative expression levels of <i>Spata33</i> and X-axis shows different development stages P2-P20. C). Expression of Spata33 protein in testes from P2 to P20. Mouse testes were individually collected from aged 2 to 20 days and were subjected to Western blot analysis with antibody against Spata33. The protein level was markedly increased from P12. Actin was used as an internal control. Molecular weight is shown on the right. All experiments were performed three times with independent individuals.</p
